Gel-Quant Electrophoresis Image Analysis Software has been developed to meet the requirements of different applications both in applied and basic sciences, especially:

       molecular biology
       medical diagnostics
       genetics
       environmental studies
       biotechnology
       microbiology

Use Gel-Quant to analyse one dimensional gel images.  After electrophoresis, scan your gel image into a bitmap format.  Once the image is digitised, use Gel-Quant to:

       provide mass and molecular weight analysis
       automatically locate the lanes on the gel image
       automatically straighten curved and distorted lanes
       select an area of interest from the image for further processing
       graphically present each lane profile separately or overlay lanes of interest
       perform Gaussian curve fitting for bands within lanes
       schematically present the processed bands (Metagel)
       arrange data from calculations made on the processed gel into a Data Table
       convert gel images from negative to positive

Results of the entire process, including the original image and analysis data, can be stored and printed.  Results can also be exported in several common formats eg, Excel.

The following is a step-by-step example of gel processing of PCR Fragments with Gel-Quant software.  Click on the icons to view a sample screen.

1. Initial gel image. 
Scanned gel images need to be in grey, 8-bit, BMP format.

2. Automated Lane Finding.
Lanes can be located either automatically or manually. Found lanes are marked with coloured borders.

3. Lane Straightening.  
An important step in gel image preparation is lane straightening. The Lane Straightening Tool allows lanes to be corrected if they are not straight or parallel to each other.

4. Removing "Smiles"
Use the deserpation tool to remove the inclination and smiles of all bands in particular lanes.  This function does not influence the position of the band medians. 

5. Standards Line Up  
Use this tool to mark the "standards" lanes.  This function ensures the known standards are identical for each standards lane.

6. Standards Setting  
Use the Standards Setting tool to match the molecular weights of standards with the positions of the corresponding bands.

7. Lane Profiles  
Click the Lane Profiles Tool to view a graphic presentation of the marked lanes.

8. Data Table
Data from calculations made on the processed gel is presented in table form.   There are several different modes of data presentation available, as well as the ability to sort data and customise column headings.  Tables can be saved and stored, and exported into other formats, including Excel.

Technical Help

AMPL Software is committed to customer service.  Unlimited, free technical support is provided to each registered owner of Gel-Quant Software.   For convenience, we provide technical support via phone, fax and email.

Contact us at:
AMPL Software Pty Ltd
Phone: (02) 9440 0269     (International Callers: +61 2 9440 0269)
Fax: (02) 9440 0271         (International Callers: +61 2 9440 0271)
Email Direct: EMail Tech Support
or
Click here to complete a technical support request form

Need more information???

Click here to request more information on Gel-Quant.

AMPL SOFTWARE PTY LTD 3 McRae Place, Turramurra NSW 2074  AUSTRALIA
Ph: (02) 9440 0269  (From overseas: +612 9440 0269)
   Fax: (02) 9440 0271 (From overseas: +612 9440 0271)

AMPL Software gratefully acknowledges the assistance of Multiplexed Biotechnologies, Inc in the creation of these pages.